自由基和其他活性種在許多心理和病理生理過程中發(fā)揮著重要作用。自由基一旦在細胞內(nèi)產(chǎn)生，就會破壞多種細胞成分，包括蛋白質(zhì)、脂質(zhì)和DNA。然而，在較低濃度下，自由基可以作為細胞信號傳導(dǎo)中的第二信使。

Enzo Life Sciences/欣博盛生物的ROS-ID® ROS/RNS detection kit可用熒光顯微鏡直接實時監(jiān)測活細胞中活性氧與活性氮（ROS/RNS）的產(chǎn)生。該試劑盒中包含三種檢測探針：NO檢測試劑（紅色，Ex/Em 650/670 nm），用于總ROS檢測的氧化應(yīng)激檢測試劑（綠色，Ex/Em 490/525 nm）和超氧化物檢測試劑（橙色，Ex/Em 550/620 nm）。試劑盒內(nèi)還包括陽性對照，綠膿菌素和L-精氨酸，它們分別是ROS和NO產(chǎn)生的常見誘導(dǎo)劑；陰性對照N-乙酰-L-半胱氨酸和c-PTIO則分別是ROS和NO的常見清除劑。通過將三種檢測探針與一組特異性抑制劑、激活劑相結(jié)合，能夠區(qū)分超氧化物、一氧化氮和過氧亞硝酸鹽。

產(chǎn)品特點

● 直接監(jiān)測活細胞中的活性氧和氮化物（ROS/RNS）

● 可區(qū)分超氧化物、一氧化氮和過氧亞硝酸鹽

● 高靈敏度、特異性和準確性，可用于活細胞研究

● 與組織培養(yǎng)基的主要成分（酚紅、FBS和BSA）兼容

● 試劑盒內(nèi)包含整套試劑，包括ROS/RNS誘導(dǎo)劑和清除劑

實驗示例

圖1. 氧化應(yīng)激檢測試劑（綠色）、超氧化物檢測試劑（藍色）和NO檢測試劑（紅色）的激發(fā)和發(fā)射光譜。

圖2. 使用ROS/RNS檢測試劑盒演示HeLa細胞中過氧亞硝酸鹽和NO生成的代表性實驗。分析表明，由于NO與超氧化物的反應(yīng)，L-精氨酸和綠膿菌素的聯(lián)合處理產(chǎn)生過氧亞硝酸鹽（綠色熒光），但很少產(chǎn)生NO（紅色熒光）。

產(chǎn)品信息

部分產(chǎn)品引用文獻

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2. Dihydroartemisinin-transferrin adducts enhance TRAIL-induced apoptosis in triple-negative breast cancer in a P53-independent and ROS-dependent manner: X. Zhou, et al.; Front. Oncol. 11, 789336 (2022)

3. Intermittent exposure of cultured endothelial cells to physiologically relevant fructose concentrations has a profound impact on nitric oxide production and bioenergetics: M.L. Fiorello, et al.; PLoS One 17, e0267675 (2022)

4. S1P (Sphingosine-1-Phosphate)-Induced Vasodilation in Human Resistance Arterioles During Health and Disease: B. Katunaric, et al.; Hypertension 79, 2250 (2022)

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6. Liposome-encapsulated bacillus calmette-guérin cell wall skeleton enhances antitumor efficiency for bladder cancer in vitro and in vivo via induction of amp-activated protein kinase: Y.M. Whang, et al.; Cancers (Basel) 12, 3679 (2020)

7. Mesenchymal stem cell conditioned medium ameliorates diabetic serum-induced insulin resistance in 3T3-L1 cells: A. Sanap, et al.; Chronic Dis. Transl. Med. 7, 47 (2020)

8. Isoorientin derived from Gentiana veitchiorum Hemsl. flowers inhibits melanogenesis by down-regulating MITF-induced tyrosinase expression: Q. Wu, et al.; Phytomedicine 57, 129 (2019)

9. Low intensity ultrasound reduces high glucose reduced nitric oxide generation in retinal pigment epithelial cells: M.B. Karmacharya, et al.; Ultrasound Med. Biol. 44, 647 (2018)

10. HEK-293 secretome attenuates kainic acid neurotoxicity through insulin like growth factor-phosphatidylinositol-3-kinases pathway and by temporal regulation of antioxidant defense machineries: C. Venugopal, et al.; Neurotoxicology 69, 189 (2017)

11. Selective enhancing effect of metal ions on mutagenicity: N. Fujii, et al.; Genes Environ. 38, 21 (2016)

12. Cell-to-cell diffusion of glucose in the mammalian heart is disrupted by high glucose. Implications for the diabetic heart: W.C. De Mello ; Exp. Cell Res. 334, 239 (2015)

13. Increased Proinflammatory Cytokine Production and Decreased Cholesterol Efflux Due to Downregulation of ABCG1 in Macrophages Exposed to Indoxyl Sulfate: K. Matsuo, et al. ; Toxins (Basel) 7, 3155 (2015)

14. Regulation of gene expression by tobacco product preparations in cultured human dermal fibroblasts: G.E. Malpass, et al.; Toxicol. Appl. Pharmacol. 279, 211 (2014)

15. Enterococcus faecalis infection causes inflammation, intracellular oxphos-independent ROS production, and DNA damage in human gastric cancer cells: J.A. Strickertsson, et al.; PLoS One 8, e63147 (2013)

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